Mitochondria and Glyoxysomes from Castor Bean Endosperm
نویسندگان
چکیده
منابع مشابه
Occurrence of RNA in glyoxysomes from castor bean endosperm.
The finding that in the endosperm of germinating castor bean, the enzymes of the glyoxylate cycle were housed in a special organelle, the glyoxysome (1, 2) raised the question of its biogenesis. We have shown that glyoxysome protein increases strikingly during the early stages of germination and concluded that rapid formation of new organelles occurs at this time (8). When the individual organe...
متن کامل6 Oxidation in Glyoxysomes from Castor Bean Endosperm
Crude particulate preparations from castor bean endosperm contain most of the /3 oxidation activity present in initial extracts. Measurements on organelles separated by sucrose density centrifugation of the crude particles show that glyoxysomes (density 1.25) contain more than 80% of the particulate /3 oxidation activity, with virtually none in the mitochondria (density 1.19). Thiolase shows a ...
متن کاملTemperature-induced Phase Changes in the Membranes of Glyoxysomes, Mitochondria, and Proplastids from Germinating Castor Bean Endosperm.
Sucrose-gradient purified mitochondria, glyoxysomes, and proplastids from germinating castor bean (Ricinus communis L.) endosperm were examined by electron-spin resonance spectroscopy. A temperature-induced phase change was demonstrated in all of these organelles, their derivative membranes, and in micelles formed from the membrane phospholipids. The apparent transition temperature of the membr...
متن کاملOrientation of electron transport activities in the membrane of intact glyoxysomes isolated from castor bean endosperm.
Intact glyoxysomes were isolated from castor bean endosperm on isometric Percoll gradients. The matrix enzyme, malate dehydrogenase, was 80% latent in the intact glyoxysomes. NADH:ferricyanide and NADH:cytochrome c reductase activities were measured in intact and deliberately broken organelles. The latencies of these redox activities were found to be about half the malate dehydrogenase latency....
متن کاملCytochemical localization of catalase activity in glyoxysomes from castor bean endosperm.
Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H(2)O(2) is distributed uniformly throughout the matrix of the organelles. Ricinosomes or dilated cisternae may be completely absent...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)83401-9